Curriculum Vitae

Research Interests

Marine microbial processes, microbial diversity, nitrogen fixation, phytoplankton ecology, biogeochemical cycling, marine disease, climate change, science communication

Education

Ph.D., College of Earth, Ocean, and Atmospheric Sciences, Oregon State University Corvallis, OR. April 2013 – present. Expected December 2016. Ocean, Earth, and Atmospheric sciences major. Advisor: Angelicque White

M.S., College of Earth, Ocean, and Atmospheric Sciences, Oregon State University  Corvallis, OR. September 2010 – March 2013. Ocean, Earth, and Atmospheric sciences major. Advisor: Ricardo Letelier. Title: A study on the effects of enhanced pCO2 on open ocean diazotrophic assemblages

B.A., Barnard College, Columbia University, New York, NY. Completed May 2010; General Biology Major

Research Experience

Graduate Research Fellow, Oregon State University, EPA STAR fellowship F13E20903, October 2014 – 2016. Project title: “Environmental Controls of the Abundance, Growth and Toxicity of the Known Shellfish Pathogen Vibrio tubiashii”

Graduate Research Assistant, Letelier/White Phytoplankton Physiology/Ecology Laboratory, Oregon State University, Corvallis, OR September 2010 – 2014. Designed and performed experiments for Master’s thesis and PhD thesis, ran samples, attended research cruises, maintained laboratory culture collection.

NSF REU Student, Strom Plankton Ecology Laboratory, Shannon Point Marine Center, Anacortes, WA June 2009 – August 2009. Completed NSF REU research project, “Experiments using Synechococcus strains WH8102 and CC9311 show no evidence for preferential grazing in natural communities or mixed cultures.”

Undergraduate Research Student, McGuire Soil Microbiology Laboratory, Barnard College, New York, NY September 2009 – August 2010. Completed undergraduate research project: The effects of coffee plantation management intensity on the community structure and function of soil fungi.

Laboratory Volunteer, Wasser Conservation Biology Laboratory, University of Washington, Seattle, WA June 2008 – August 2008. Performed DNA extractions, DNA purifications, and Polymerase Chain Reactions; analyzed DNA microsatellite sequence data.

Teaching Experience

  • Graduate Teaching Assistant, Principles of Biology 213: Genetics, Ecology, and Evolution, March 2012—June 2012 and March 2013 – June 2013.
  • Co-teacher for 2nd grade science class, September 2009 – December 2009. Emily Dickenson School, New York City.
  • Teaching coursework: “Science in the City” (elementary science education), Barnard College, 2009; “Communicating Ocean Sciences” (informal education), Oregon State University, 2012; “Making your Science Matter” (informal education), Oregon State University, 2014.
  • Guest lecturer for high school AP Biology class: “Ocean Acidification and Marine Microbes.” June 2 2010.

Honors and awards

  • EPA STAR Awardee, Environmental Protection Agency, May 2014. Title: “Environmental Controls of the Abundance, Growth and Toxicity of the Known Shellfish Pathogen Vibrio tubiashii”
  • Agouron Summer Microbial Oceanography Course Participant, May – June 2014
  • Honorable Mention, NSF Graduate Research Fellowship Program, 2012.
  • Recipient, Herbert Maule Richards Fund Grant, 2010. Research grant funded soil microbiology fieldwork conducted in Malaysia, August 2010.

Relevant Skills

  • Experienced in DNA and RNA extractions and quantification, clone library sequencing, sequence editing, high throughput sequencing, bioinformatic analysis (amplicon and metagenome), gel electrophoresis, polymerase chain reaction, acetylene reduction assays, 14C, 15N2 and 13C incubations, fluorometry, flow cytometry, bacterial and protist slide preparation and cell counts, filtration, Winkler titrations, acid-base titrations, water sample collection, shipboard incubation experiments, grazer feeding experiments, extracellular enzyme assays, culture maintenance, sterile technique, microscopy
  • Proficient using gas chromatograph, Turner fluorometer, Pulse Amplitude Modulation fluorometer, spectrofluorometer, thermocycler, membrane inlet mass spectrometer, C/H/N analyzer, filtration rig, flow cytometer, LI-COR, pH meter, gas cylinder, regulator, and flow meter, peristaltic pump, micropipettor, Conductivity, Temperature, Depth (CTD) sensor, plankton net, plate reader, autoclave, microscope (epifluorescent, light, dissecting)
  • Proficient using Microsoft Excel, Microsoft Word, Microsoft Powerpoint, Endnote, MATLAB, R Statistics, Unix, CO2sys

Refereed Articles:

  • Gradoville, MR, White, AE, Letelier, RM. Physiological response of Crocosphaera watsonii to enhanced and fluctuating carbon dioxide conditions. PLoS ONE 9(10): e110660. doi:10.1371/journal.pone.0110660
  • Gradoville, MR, White, AE, Böttjer, D, Church, MJ, Letelier, RM (2014) Diversity trumps acidification: Lack of evidence for carbon dioxide enhancement of Trichodesmium community nitrogen or carbon fixation at Station ALOHA. Limnology and Oceanography 59: 645–659.
  • Wood-Charlson, EM, Bender, SJ, Bruno, BC, Diaz, JM, Gradoville, MR, Loury, E, Viviani, DA. 2015. Translating Science Into Stories. ASLO Bulletin August 2015, 1-4.
  • McGuire, KL, D’Angelo, H, Brearley, FQ, Gedallovich, SM, Babar, N, Yang, N. Gillikin, CM, Gradoville, MR, Bateman, C, Turner, BL, Mansor, P, Leff, JW, Fierer, N. 2015. Responses of soil fungi to logging and oil palm agriculture in Southeast Asian tropical forests. Microbial Ecology 69: 733-747

Scientific presentations:

  • Gradoville, MR, Letlier, RM, Bombar, D, White, AE. Searching for aphotic marine nitrogen fixation in the Pacific Northwest and the South Pacific. Joint Aquatic Sciences Meeting, May 2014, Portland, OR, oral presentation.
  • Gradoville, MR, White AE, Böttjer D, Church MJ, Letelier RM (2014) Diversity trumps acidification: No CO2 enhancement of N2 fixation by the Trichodesmium community at Station ALOHA. Ocean Sciences Meeting, February 2014, Honolulu Hawaii, oral presentation.
  • D’Angelo, H, McGuire, KL, Essene, A, Massmann, A, Mansor, P, Yang, N, Gillikin, CM, Gradoville, MR, Gedallovich, SM, Babar, N. Turner, BL, Merrer, D, Gilbert, JA, Peay, K, Leff, J, Fierer, N. Simultaneous analysis of fungal and bacterial composition across a soil edaphic gradient in Southeast Asian dipterocarp rain forest. 99th Ecological Society of America Annual Convention, August 2014, Sacramento, CA, poster presentation.
  • Gradoville, MR, White, AE, Zirbel, MJ, Bottjer, D, Church, MJ, Letelier, RM. 2012. Metabolic response of Trichodesmium and Crocosphaera to pCO2 perturbations on multiple time scales. Ocean Sciences Meeting, Salt Lake City, Utah, February 2012, poster presentation.
  • Gradoville, MR, Watkins-Brandt, KS, White, AE, Church, MJ, Letelier, RM. 2011. Comparison of N2 fixation response by Trichodesmium to pCO2 perturbations using bas bubbling and acid/base approaches. ASLO Aquatic Sciences Meeting, San Juan, Puerto Rico, February 2011, oral presentation.
  • McGuire, KL, Philpott, SM, Gradoville, MR, Gillikin, C. Coffee from the ground up: rustic coffee plantations provide better habitats for soil fungal communities than sun coffee plantations. Argonne National Laboratory Soil Metagenomics Workshop, Chicago, Illinois, October 2011, poster presentation.

Activities, Outreach and Service

  • Promotion and Tenure Committee Student Committee Member, 2014 – 2015.
  • Center for Microbial Oceanography: Research and Education Professional Development Organizing Committee (PDOC) member, March 2013 – April 2014.
  • Student Advisory Committee Social Coordinator, August 2013 – August 2014.
  • Science outreach activity leader: Teach and lead marine science outreach activities designed to teach children about microbial oceanography and climate change. Select outreach events:
    • Underwater Exploration – Hands on Activities about our Fascinating Ocean, Boy Scouts of America, Hoover Elementary, February 4, 2014 and April 15 2015
    • OceanFEST (Families Exploring Science Together), Hoover Elementary, April 20, 2012; Franklin Elementary, April 6, 2012; Jefferson Elementary, February 22, 2011
  • NSF Week-Long Professional Development Workshop Participant, August 2015
  • Center for Microbial Oceanography: Research and Education professional development training events
    • “Teaching Methods and Strategies” coordinator and participant, January 14, 2015
    • “Communicating your Science: A Professional Necessity” coordinator and participant, August 27, 2014
    • “Mentoring Workshop” coordinator and participant, July 15, 2014
    • “Effective Communication and Team-Building” coordinator and participant, February 23, 2014
    • “Proposal Writing Workshop” coordinator and participant, July 17, 2013
    • “Family-Career-Life Balance Workshop” coordinator and participant, July 2, 2013
    • “Workshop on Careers and Professional Skills” participant, October 26, 2012
    • “COMPASS Science Communication Training” participant, May 23, 2011

Oceanographic Field Experience

Cruise Vessel Year Days at Sea
Zooplankton Sampling Elahka 2015 1
Endurance Oceanus 2014 4
Agouron Summer Course Kilo Moana 2014 7
OC1404D Student Cruise Oceanus 2014 4
HOE BOE Kilo Moana 2014 13
NOAA West Coast Ocean Acidification NOAA Fairweather and Pt. Sur 2013 16
HOE DYLAN VII Kilo Moana 2012 10
KM1110 Kilo Moana 2011 12

Total                     67 days

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